« Back to Directory

Stein, Lisa

Bio

EDUCATION:



1998: Ph.D., Molecular and Cellular Biology, Oregon State University



1991: B.A., Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Magna cum laude, 1991


 


PROFESSIONAL EXPERIENCE:



2016-present: Professor, Department of Biological Sciences, University of Alberta, Edmonton, Alberta



2008-2016: Associate Professor, Department of Biological Sciences, University of Alberta, Edmonton, Alberta



2001-2008: Assistant Professor and Assistant Environmental Microbiologist, Department of Environmental Sciences, University of California, Riverside



1998-2001: Postdoctoral Scholar, Division of Geological and Planetary Sciences, California Institute of Technology and Center for Life Detection, Jet Propulsion Laboratory, NASA


1993-1998: Graduate Research Assistant, Molecular and Cellular Biology Graduate Program, Oregon State University, Corvallis



1991-1993: Professional Research Assistant, Department of Chemistry and Biochemistry, University of Colorado, Boulder


 


SELECT PUBLICATIONS:


Kits, K.D., C.J. Sedlacek, E.V. Lebedeva, P. Han, A. Bulaev, P. Pjevac, A. Daebeler, S. Romano, M. Albertsen, L.Y. Stein, H. Daims, M. Wagner. 2017. Kinetic analysis of a complete nitrifier reveals competitiveness in oligotrophic habitats. Nature. 549:269-272. doi: 10.1038/nature23679. Epub 2017 Aug 23.


Kozlowski, J.A., M. Stieglmeier, C. Schleper, M.G. Klotz and L.Y. Stein. 2016. Pathways and key intermediates required for obligate ammonia-dependent chemolithotrophy. ISME J. 10:1836-1845.


Stein, L.Y. and M.G. Klotz. 2016. The Nitrogen Cycle. Curr. Biol. 26:R94–R98. http://dx.doi.org/10.1016/j.cub.2015.12.021


Kits, K.D., D.J. Campbell, A. Remus-Rosana, L.Y. Stein. Hypoxia and nitrite control denitrification activity and expression of denitrification genes in the obligate methanotroph Methylomicrobium album strain BG8. 2015. Front. Microbiol. 6:1072. doi: 10.3389/fmicb.2015.01072


Kits, K.D., M.G. Klotz and L.Y. Stein. 2015. Methane oxidation coupled to nitrate reduction under hypoxia by the gammaproteobacterium Methylomonas denitrificans sp. nov. Type Strain FJG1. Environ. Microbiol. doi: 10.1111/1462-2920.12772.


Cua, L.S. and L.Y. Stein. 2014. Characterization of denitrifying activity by the alphaproteobacterium, Sphingomonas wittichii RW1. Front. Microbiol. 5:404. doi: 10.3389/fmicb.2014.00404


Kozlowski, J.A., J. Price and L.Y. Stein. 2014. Revision of N2O-producing pathways in the ammonia-oxidizing bacterium, Nitrosomonas europaea ATCC 19718. Appl. Environ. Microbiol. 80:4930-4935. doi:10.1128/AEM.01061-14


Prosser, J.I., I. Head and L.Y. Stein. 2014. The family Nitrosomonaceae. In The Prokaryotes, Alphaproteobacteria and Betaproteobacteria 4th ed. E. Rosenberg, E.F. Delong, S. Lory, E. Stackebrandt & F. Thompson (eds). Heidelberg: Springer Science. pp. 901-918


Stein, L.Y., M.A. Campbell and M.G. Klotz. 2013. Energy-mediated versus ammonium-regulated gene expression in the obligate ammonia-oxidizing bacterium, Nitrosococcus oceani. Front. Microbiol. 4:277. doi:10.3389/fmicb. 2013.00277


Kartal, B. H.J.C.T. Wessels, E. van der Biezen, K.-J. Francoijs, M.S.M. Jetten, M.G. Klotz, and L.Y. Stein. 2012. Effects of nitrogen dioxide and anoxia on global gene and protein expression in long-term continuous cultures of Nitrosomonas eutropha C91. Appl. Environ. Microbiol. 78:4788-4794. 



 


SYNERGISTIC ACTIVITIES



2016: Invited participant for ASM-AGU Collaborative Colloquium on the Effects of Climate Change on Microbial Ecosystems


2015: Invited chair for Nitrification section of NSF INFEWS workshop on the Nitrogen Cycle


2015: Chair and Organizer of 4th International Conference on Nitrification and Related Processes (ICoN4)


2011-present: Member, Editorial Board, Applied and Environmental Microbiology, 2010-present: Member, Editorial Board, Environmental Microbiology



2010-2016: Specialty Chief Editor, Frontiers in Terrestrial Microbiology



2016-2017: Associate Editor, Frontiers in Microbial Physiology & Metabolism


2010-2011: Co-Editor for “Nitrification and Related Processes.” Meth. Enzymol. Vol 496


2010: Founder of Organization for Methanotroph Genome Analysis (OMeGA)



2010, 2013, 2016: US Dept. of Energy grant review panel for Systems Biology Carbon Cycling


2009-2013: Nitrification Network Steering Committee



 


COLLABORATORS AND OTHER AFFILIATIONS



Collaborators: Martin G. Klotz (Wash State Univ.), Dominic Sauvageau (Univ. Alberta), Marina G. Kalyuzhnaya (San Diego State), Michael Wagner (Univ. Vienna), Holger Daims (Univ. Vienna), Stéphane Vuilleumier (Univ. Strasbourg), Christa Schleper (Univ. Vienna), Jeanette Norton (Utah State Univ.), James I. Prosser (Univ. Aberdeen)


Advisors: Dr. Daniel J. Arp (PhD), Dr. Kenneth H. Nealson (Postdoc)



Completed Students: Dr. Györgyi Nyerges (PhD), Dr. Lynnie Cua (PhD), Jessica Kozlowski (PhD), Kerim D. Kits (PhD), Miyuki Yamamoto (MSc), Jennifer Lobel (MSc), Mohammad Ghashghavi (MSc), Misha Miazga-Rodriguez (MSc)


Current Students: Catherine Tays (PhD), Shibashis Das (MSc), Marc Waddingham (MSc), Phillip Sun (MSc), Scott Sugden (MSc)

Stein, Lisa

Publications, Activities, and Awards

  • The long-term relationship between microbial metabolism and greenhouse gases
  • A multi-level “-omics” approach to the study of the growth regulation of methanotrophs
  • A multi-level “-omics” approach to the study of the growth regulation of methanotrophs. Future Energy Systems Research Symposium.
  • Adaptation of Methylobacterium extorquens AM1 to growth at low pH
  • Adaptive Evolution of Methylomicrobium album BG8 on a low pH medium
  • Advanced bioreactor operation improve methanol bioconversion in Methylotuvimicrobium buryatense 5GB1C
  • An overlooked influence of reactive oxygen species on ammonia-oxidizing microbial communities in redox-fluctuating aquifers
  • ASM Microbe- Session co-convener
  • Bacteriohemerythrin at the Crossroads; Hemerythrin from Methylomonas denitrificans FJG1 sets methanotrophs apart
  • Bacteriophages as a sustainable and reliable means of product recovery
  • Big Solutions from the Smallest Lifeforms
  • Bioconversion of methane emissions into biofuels and biofuel precursors
  • Bioconversion of methane to biodegradable plastic
  • Bioconversion of methane to biodegradable plastic
  • Bioconversion of methane to biodegradable plastic: Metabolic route from greenhouse gas to green product
  • Bioconversion of Methane to Biopolymers: An Ecofriendly and Sustainable Approach to Mitigate Environmental Problems
  • Bioconversion of single-carbon substrates in fed-batch cultures.
  • Bioconversion of waste methane emissions to value-added biofuels and biomaterials
  • Biomass production of Methylotuvimicrobium buryatense 5GB1C using two types of advanced bioreactor operations
  • Biomass: Microbial bioconversion to fuels and products
  • Biotechnology for a Sustainable Environment
  • Can Pink Bacteria Help Mitigate Methane Emissions
  • Can pink bacteria help mitigate methane emissions?
  • Cell lysis and enhanced product recovery from methanotrophic bacteria
  • Cell lysis and enhanced product recovery from methanotrophic bacteria. Future Energy Systems Research Symposium.
  • Changing Methane Paradigms
  • Characterizing Outer Membrane Vesicles in Methanotrophic Bacteria
  • Chinese Assoc. for Microbial Ecology
  • Climate Change Mitigation and Adaptation in Agriculture: what can biotechnology do?
  • Combined effects of carbon and nitrogen source to optimize growth of proteobacterial methanotrophs
.
  • Complete genome sequence of Methylomonas denitrificans strain FJG1, an obligate aerobic methanotroph that can couple methane oxidation to denitrification
  • Converting Fugitive Methane Emissions into Value-Added Products
  • Development of a Methanotroph Chassis: Genetic engineering of Methylomicrobium album BG8 for production of isoprenoids from C1 compounds
  • Development of a methanotroph chassis: genetic engineering of Methylomicrobium album BG8 for production of value-added products.
  • Development of a nutrient supply-based process for industrial-scale mitigation of methane emissions by bacteria
  • Development of a plasmid-based gene expression system for methanotrophs
  • Diverse bacteriohemerythrin genes of Methylomonas denitrificans FJG1 provide insight into the survival and activity of proteobacterial methanotrophs under hypoxia
  • Effect of copper on the growth of methanotrophic bacteria in a fed-batch process
  • Effect of isoprenoids on the growth of industrially relevant methanotrophs
  • Energy Solutions
  • Examining Protein Cargo and the Potential Physiological Mechanisms of Outer Membrane Vesicles (OMVs) in Methanotrophic Bacteria
  • Free Shipping? Exploring the Protein Cargo Content of Outer Membrane Vesicles (OMVs) in Methanotrophic Bacteria
  • Genomic taxonomy of the gammaproteobacterial methanotrophs (Methylococcales) calls for the reclassification of members within this order
  • Gold College --lab training for student
  • How Can Agrigenomics Help to Address Climate Change?
  • Identification and characterization of lysogenized phages in methanotrophic
  • Identification and characterization of lysogenized phages in methanotrophic bacteria
  • Impact of substrates on development of industrial processes for methylotrophs.
  • Improving Extraction of Biodegradable Plastics from Bacteria
  • In vivo and in vitro quantification of polyhydroxybutyrate (PHB) in bacteria
  • In vivo and in vitro quantification of polyhydroxybutyrate (PHB) in bacteria
  • Interactions of PHB and switchable hydrophilicity solvents: A more sustainable approach to PHB recovery
  • Interactions of PHB and switchable hydrophilicity solvents: A more sustainable approach to PHB recovery
  • Introductory microbiology demonstration
  • Isolating Outer Membrane Vesicles from Methanotrophic Bacteria
  • Lysogenized phages of methanotrophic bacteria show a broad genetic diversity
  • Metabolic mapping of Methylomicrobium album strain BG8
  • Metabolome profiles of the alphaproteobacterial methanotroph Methylocystis sp. Rockwell in response to carbon and nitrogen source
  • Methane Bioconversions
  • Methane-munching microbes and the carbon footprint of climate change
  • Methanol bioconversion in Methylotuvimicrobium buryatense 5GB1C through self-cycling fermentation
  • Microbes & Climate Change
  • Microbial methane munchers offer a shield from the scorch
  • Microbiome of the freshwater sponge Ephydatia muelleri shares compositional and functional similarities with marine sponges
  • Optimization of bacterial growth and polyhydroxybutyrate (PHB) yield by Methylocystis sp. Rockwell
  • Optimization of growth and polyhydroxybutyrate (PHB) production from Methylocystis sp. strain Rockwell
  • Optimization of Methane Feed and N:C Ratio for Biomass and Polyhydroxybutyrate Production by the Alphaproteobacterial Methanotroph Methylocystis sp. Rockwell
  • Optimizing methanotroph bioproduction through growth strategies and strain adaptation
  • Optimizing methanotroph biotechnology through growth strategies
  • Optimizing methanotroph biotechnology through growth strategies and strain adaptation.
  • Permafrost Dialogues
  • Phylogenomic analysis of the gammaproteobacterial methanotrophs (order Methylococcales) calls for the reclassification of members at the genus and species levels
  • Positron-emitting tracers spatially resolve unexpected biogeochemical relationships linked with methane oxidation in Arctic soils
  • Reduction of greenhouse gas emissions through the bioconversion of methane from Albertan industrial sectors.
  • Science for a Sustainable Planet
  • Sea-ice algae are losing their homes and the ocean is at risk of starving
  • Substrate-dependent growth variability across model methanotrophic strains
  • Sustainable Solutions from the Smallest Organisms
  • Switchable solvents for the reversible dissolution of poly(3-hydroxybutyrate)
  • Systematic Development of a Methanotroph chassis for the production of value-added products from C1 waste of Agriculture and Forestry: Predictive modeling and genetic engineering of Methylomicrobium album BG8
  • Systematic Development of a Methanotroph Chassis for transforming wastewater methane emissions to value added products: Predictive modelling and genetic engineering of Methylomicrobium album BG8
  • The application of a switchable solvent to the extraction of polyhydroxybutyrate from methanotrophic bacteria.
  • The Application of Switchable Solvents to the Extraction of Bioproducts from Methanotrophic Bacteria
  • The Application of Switchable Solvents to the Extraction of Bioproducts from Methanotrophic Bacteria
  • The combined effect of methane and methanol on growth and PHB production in the alphaproteobacterial methanotroph Methylocystis sp. Rockwell.
  • The double life of Methanoperedens
  • The hunt for viruses that infect methane consuming bacteria
  • The influence of self-cycling fermentation long-and short-cycle schemes on Saccharomyces cerevisiae and Escherichia coli
  • The Ins and Outs of OMVs; Examining the Contents and Causes of Outer Membrane Vesicles (OMVs) in Methanotrophic Bacteria
  • The Ins and Outs of OMVs; Examining the Contents and Causes of Outer Membrane Vesicles (OMVs) in Methanotrophic Bacteria
  • Transcriptomic analysis of synchrony and productivity in self-cycling fermentation of engineered yeast producing shikimic acid
  • Transcriptomic and metabolomic responses to carbon and nitrogen sources in Methylomicrobium album BG8
  • USchool Hands-on demonstration
  • Uschool Hands-on demonstration