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Stein, Lisa



1998: Ph.D., Molecular and Cellular Biology, Oregon State University

1991: B.A., Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Magna cum laude, 1991



2016-present: Professor, Department of Biological Sciences, University of Alberta, Edmonton, Alberta

2008-2016: Associate Professor, Department of Biological Sciences, University of Alberta, Edmonton, Alberta

2001-2008: Assistant Professor and Assistant Environmental Microbiologist, Department of Environmental Sciences, University of California, Riverside

1998-2001: Postdoctoral Scholar, Division of Geological and Planetary Sciences, California Institute of Technology and Center for Life Detection, Jet Propulsion Laboratory, NASA

1993-1998: Graduate Research Assistant, Molecular and Cellular Biology Graduate Program, Oregon State University, Corvallis

1991-1993: Professional Research Assistant, Department of Chemistry and Biochemistry, University of Colorado, Boulder



Kits, K.D., C.J. Sedlacek, E.V. Lebedeva, P. Han, A. Bulaev, P. Pjevac, A. Daebeler, S. Romano, M. Albertsen, L.Y. Stein, H. Daims, M. Wagner. 2017. Kinetic analysis of a complete nitrifier reveals competitiveness in oligotrophic habitats. Nature. 549:269-272. doi: 10.1038/nature23679. Epub 2017 Aug 23.

Kozlowski, J.A., M. Stieglmeier, C. Schleper, M.G. Klotz and L.Y. Stein. 2016. Pathways and key intermediates required for obligate ammonia-dependent chemolithotrophy. ISME J. 10:1836-1845.

Stein, L.Y. and M.G. Klotz. 2016. The Nitrogen Cycle. Curr. Biol. 26:R94–R98. http://dx.doi.org/10.1016/j.cub.2015.12.021

Kits, K.D., D.J. Campbell, A. Remus-Rosana, L.Y. Stein. Hypoxia and nitrite control denitrification activity and expression of denitrification genes in the obligate methanotroph Methylomicrobium album strain BG8. 2015. Front. Microbiol. 6:1072. doi: 10.3389/fmicb.2015.01072

Kits, K.D., M.G. Klotz and L.Y. Stein. 2015. Methane oxidation coupled to nitrate reduction under hypoxia by the gammaproteobacterium Methylomonas denitrificans sp. nov. Type Strain FJG1. Environ. Microbiol. doi: 10.1111/1462-2920.12772.

Cua, L.S. and L.Y. Stein. 2014. Characterization of denitrifying activity by the alphaproteobacterium, Sphingomonas wittichii RW1. Front. Microbiol. 5:404. doi: 10.3389/fmicb.2014.00404

Kozlowski, J.A., J. Price and L.Y. Stein. 2014. Revision of N2O-producing pathways in the ammonia-oxidizing bacterium, Nitrosomonas europaea ATCC 19718. Appl. Environ. Microbiol. 80:4930-4935. doi:10.1128/AEM.01061-14

Prosser, J.I., I. Head and L.Y. Stein. 2014. The family Nitrosomonaceae. In The Prokaryotes, Alphaproteobacteria and Betaproteobacteria 4th ed. E. Rosenberg, E.F. Delong, S. Lory, E. Stackebrandt & F. Thompson (eds). Heidelberg: Springer Science. pp. 901-918

Stein, L.Y., M.A. Campbell and M.G. Klotz. 2013. Energy-mediated versus ammonium-regulated gene expression in the obligate ammonia-oxidizing bacterium, Nitrosococcus oceani. Front. Microbiol. 4:277. doi:10.3389/fmicb. 2013.00277

Kartal, B. H.J.C.T. Wessels, E. van der Biezen, K.-J. Francoijs, M.S.M. Jetten, M.G. Klotz, and L.Y. Stein. 2012. Effects of nitrogen dioxide and anoxia on global gene and protein expression in long-term continuous cultures of Nitrosomonas eutropha C91. Appl. Environ. Microbiol. 78:4788-4794. 



2016: Invited participant for ASM-AGU Collaborative Colloquium on the Effects of Climate Change on Microbial Ecosystems

2015: Invited chair for Nitrification section of NSF INFEWS workshop on the Nitrogen Cycle

2015: Chair and Organizer of 4th International Conference on Nitrification and Related Processes (ICoN4)

2011-present: Member, Editorial Board, Applied and Environmental Microbiology, 2010-present: Member, Editorial Board, Environmental Microbiology

2010-2016: Specialty Chief Editor, Frontiers in Terrestrial Microbiology

2016-2017: Associate Editor, Frontiers in Microbial Physiology & Metabolism

2010-2011: Co-Editor for “Nitrification and Related Processes.” Meth. Enzymol. Vol 496

2010: Founder of Organization for Methanotroph Genome Analysis (OMeGA)

2010, 2013, 2016: US Dept. of Energy grant review panel for Systems Biology Carbon Cycling

2009-2013: Nitrification Network Steering Committee



Collaborators: Martin G. Klotz (Wash State Univ.), Dominic Sauvageau (Univ. Alberta), Marina G. Kalyuzhnaya (San Diego State), Michael Wagner (Univ. Vienna), Holger Daims (Univ. Vienna), Stéphane Vuilleumier (Univ. Strasbourg), Christa Schleper (Univ. Vienna), Jeanette Norton (Utah State Univ.), James I. Prosser (Univ. Aberdeen)

Advisors: Dr. Daniel J. Arp (PhD), Dr. Kenneth H. Nealson (Postdoc)

Completed Students: Dr. Györgyi Nyerges (PhD), Dr. Lynnie Cua (PhD), Jessica Kozlowski (PhD), Kerim D. Kits (PhD), Miyuki Yamamoto (MSc), Jennifer Lobel (MSc), Mohammad Ghashghavi (MSc), Misha Miazga-Rodriguez (MSc)

Current Students: Catherine Tays (PhD), Shibashis Das (MSc), Marc Waddingham (MSc), Phillip Sun (MSc), Scott Sugden (MSc)

Stein, Lisa

Publications, Activities, and Awards

  • A multi-level “-omics” approach to the study of the growth regulation of methanotrophs
  • A multi-level “-omics” approach to the study of the growth regulation of methanotrophs. Future Energy Systems Research Symposium.
  • Adaptive Evolution of Methylomicrobium album BG8 on a low pH medium
  • Bioconversion of methane emissions into biofuels and biofuel precursors
  • Bioconversion of single-carbon substrates in fed-batch cultures.
  • Cell lysis and enhanced product recovery from methanotrophic bacteria
  • Cell lysis and enhanced product recovery from methanotrophic bacteria. Future Energy Systems Research Symposium.
  • Combined effects of carbon and nitrogen source to optimize growth of proteobacterial methanotrophs
  • Complete genome sequence of Methylomonas denitrificans strain FJG1, an obligate aerobic methanotroph that can couple methane oxidation to denitrification
  • Development of a Methanotroph Chassis: Genetic engineering of Methylomicrobium album BG8 for production of isoprenoids from C1 compounds
  • Development of a methanotroph chassis: genetic engineering of Methylomicrobium album BG8 for production of value-added products.
  • Development of a plasmid-based gene expression system for methanotrophs
  • Effect of copper on the growth of methanotrophic bacteria in a fed-batch process
  • Genomic taxonomy of the gammaproteobacterial methanotrophs (Methylococcales) calls for the reclassification of members within this order
  • Identification and characterization of lysogenized phages in methanotrophic
  • Identification and characterization of lysogenized phages in methanotrophic bacteria
  • Impact of substrates on development of industrial processes for methylotrophs.
  • Introductory microbiology demonstration
  • Metabolic mapping of Methylomicrobium album strain BG8
  • Optimization of bacterial growth and polyhydroxybutyrate (PHB) yield by Methylocystis sp. Rockwell
  • Optimizing methanotroph bioproduction through growth strategies and strain adaptation
  • Optimizing methanotroph biotechnology through growth strategies and strain adaptation.
  • Phylogenomic analysis of the gammaproteobacterial methanotrophs (order Methylococcales) calls for the reclassification of members at the genus and species levels
  • Reduction of greenhouse gas emissions through the bioconversion of methane from Albertan industrial sectors.
  • Substrate-dependent growth variability across model methanotrophic strains
  • Systematic Development of a Methanotroph chassis for the production of value-added products from C1 waste of Agriculture and Forestry: Predictive modeling and genetic engineering of Methylomicrobium album BG8
  • The application of a switchable solvent to the extraction of polyhydroxybutyrate from methanotrophic bacteria.
  • The hunt for viruses that infect methane consuming bacteria